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fluorescein isothiocyanate fitc conjugated gsl ib4 (Vector Laboratories)

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Vector Laboratories fluorescein isothiocyanate fitc conjugated gsl ib4
$Detection of fluorescently labeled dextran leaking from cardiac capillaries A. Three fractionations by fluorescence intensity: background (BG), extra-capillary (BG-BG x a), intra-capillary (>BG x a). B. Methods for calculating background. <t>FITC-conjugated</t> <t>GSL-IB4</t> was injected into the tail vein 5 min after PBS injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue sections were cut transversely at 10 μm using the cryostat, and the sections were mounted onto slides. The slides were dried thoroughly and observed under a fluorescence microscope. Twenty 150 μm × 150 μm per 1 section were cut out from the left ventricular wall, where capillaries were observed parallel to the myocardial layer, using LAS X software (Leica), and saved as TIFF (922 x 922 pixels; 22461.544 μm 2 /1 area). Twenty areas per section, three sections per mouse, and three (young) or two (aged) female mice were analyzed, resulting in a total of 180 or 120 images ( see Materials and Methods section for details ). Scale bar: 1 mm. C. Methods for calculating the amount of fluorescently labeled dextran leaked from capillaries. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after TD 40 injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue cut sections were prepared in the same manner as above. Fluorescence images of dextran and GSL-IB4 are imported into ImageJ separately, converted to grayscale, and binarized by setting the threshold value for each. The ratio of fluorescence-detected areas to total area was calculated from the acquired fluorescent images. Three sections per mouse, with three mice per month, were analyzed for a total of 180 images. Scale bar: 50 μm.$
Fluorescein Isothiocyanate Fitc Conjugated Gsl Ib4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescein isothiocyanate fitc conjugated gsl ib4/product/Vector Laboratories
Average 95 stars, based on 323 article reviews

fluorescein isothiocyanate fitc conjugated gsl ib4 - by Bioz Stars, 2026-03

95/100 stars

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1) Product Images from "Assessment of permeability in deep tissue capillaries using a new method reflects the nutrient supply status in a healthy heart"

Article Title: Assessment of permeability in deep tissue capillaries using a new method reflects the nutrient supply status in a healthy heart

Journal: Regenerative Therapy

doi: 10.1016/j.reth.2025.10.005

$Detection of fluorescently labeled dextran leaking from cardiac capillaries A. Three fractionations by fluorescence intensity: background (BG), extra-capillary (BG-BG x a), intra-capillary (>BG x a). B. Methods for calculating background. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after PBS injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue sections were cut transversely at 10 μm using the cryostat, and the sections were mounted onto slides. The slides were dried thoroughly and observed under a fluorescence microscope. Twenty 150 μm × 150 μm per 1 section were cut out from the left ventricular wall, where capillaries were observed parallel to the myocardial layer, using LAS X software (Leica), and saved as TIFF (922 x 922 pixels; 22461.544 μm 2 /1 area). Twenty areas per section, three sections per mouse, and three (young) or two (aged) female mice were analyzed, resulting in a total of 180 or 120 images ( see Materials and Methods section for details ). Scale bar: 1 mm. C. Methods for calculating the amount of fluorescently labeled dextran leaked from capillaries. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after TD 40 injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue cut sections were prepared in the same manner as above. Fluorescence images of dextran and GSL-IB4 are imported into ImageJ separately, converted to grayscale, and binarized by setting the threshold value for each. The ratio of fluorescence-detected areas to total area was calculated from the acquired fluorescent images. Three sections per mouse, with three mice per month, were analyzed for a total of 180 images. Scale bar: 50 μm.$
Figure Legend Snippet: Detection of fluorescently labeled dextran leaking from cardiac capillaries A. Three fractionations by fluorescence intensity: background (BG), extra-capillary (BG-BG x a), intra-capillary (>BG x a). B. Methods for calculating background. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after PBS injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue sections were cut transversely at 10 μm using the cryostat, and the sections were mounted onto slides. The slides were dried thoroughly and observed under a fluorescence microscope. Twenty 150 μm × 150 μm per 1 section were cut out from the left ventricular wall, where capillaries were observed parallel to the myocardial layer, using LAS X software (Leica), and saved as TIFF (922 x 922 pixels; 22461.544 μm 2 /1 area). Twenty areas per section, three sections per mouse, and three (young) or two (aged) female mice were analyzed, resulting in a total of 180 or 120 images ( see Materials and Methods section for details ). Scale bar: 1 mm. C. Methods for calculating the amount of fluorescently labeled dextran leaked from capillaries. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after TD 40 injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue cut sections were prepared in the same manner as above. Fluorescence images of dextran and GSL-IB4 are imported into ImageJ separately, converted to grayscale, and binarized by setting the threshold value for each. The ratio of fluorescence-detected areas to total area was calculated from the acquired fluorescent images. Three sections per mouse, with three mice per month, were analyzed for a total of 180 images. Scale bar: 50 μm.

Techniques Used: Labeling, Fluorescence, Injection, Microscopy, Software

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Journal: Regenerative Therapy

Article Title: Assessment of permeability in deep tissue capillaries using a new method reflects the nutrient supply status in a healthy heart

doi: 10.1016/j.reth.2025.10.005

Figure Lengend Snippet: Detection of fluorescently labeled dextran leaking from cardiac capillaries A. Three fractionations by fluorescence intensity: background (BG), extra-capillary (BG-BG x a), intra-capillary (>BG x a). B. Methods for calculating background. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after PBS injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue sections were cut transversely at 10 μm using the cryostat, and the sections were mounted onto slides. The slides were dried thoroughly and observed under a fluorescence microscope. Twenty 150 μm × 150 μm per 1 section were cut out from the left ventricular wall, where capillaries were observed parallel to the myocardial layer, using LAS X software (Leica), and saved as TIFF (922 x 922 pixels; 22461.544 μm 2 /1 area). Twenty areas per section, three sections per mouse, and three (young) or two (aged) female mice were analyzed, resulting in a total of 180 or 120 images ( see Materials and Methods section for details ). Scale bar: 1 mm. C. Methods for calculating the amount of fluorescently labeled dextran leaked from capillaries. FITC-conjugated GSL-IB4 was injected into the tail vein 5 min after TD 40 injection, and another 5 min later, mice were sacrificed, the cardiac tissue was removed, and promptly frozen with isopentane onto dry ice. Frozen tissue cut sections were prepared in the same manner as above. Fluorescence images of dextran and GSL-IB4 are imported into ImageJ separately, converted to grayscale, and binarized by setting the threshold value for each. The ratio of fluorescence-detected areas to total area was calculated from the acquired fluorescent images. Three sections per mouse, with three mice per month, were analyzed for a total of 180 images. Scale bar: 50 μm.

Article Snippet: To visualize cardiac endothelial cells, fluorescein isothiocyanate (FITC) -conjugated GSL-IB4 (#FL-1201; Vector Laboratories Inc., Burlingame, CA, USA) was injected into the tail vein 5 min after TD 40 injection under anesthesia (1 mg/ml, 50 μl/10 g).

Techniques: Labeling, Fluorescence, Injection, Microscopy, Software